Composite
Part:BBa_K4408005
Designed by: Leyu Xu Group: iGEM22_Worldshaper-NJBIOX (2022-09-29)
thl modified by oligopeptide SpyCatcher
This part is the coding region of thl enzyme combined with genes coding for SpyCatcher oligopeptide and Linker 1. It is constructed by thl (BBa_K3443003), SpyCatcher (BBa_K3329016) and Linker 1(BBa_K1583012) sequences. It can be bounded with SpyTag-Linker 1-hbd (BBa_K4408006) to enhance the catalysis of acetyl-CoA into 3-hydroxybutytyl-CoA by thl and hbd enzymes in the metabolic pathway of Clostridium tyrobutyricum.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1076
Illegal SapI.rc site found at 900
[edit]
Categories
Parameters
None |